利用 NovaSeq 6000 测序技术，可以对功能基因进行保守区域多样性进行研究。通常选取功能基因片段应在500bp以内，以确保可以测通并保证拼接质量。我们已经做过多个功能基因多样性分析，有完善的注释系统数据库。我们基于fungene（http://fungene.cme.msu.edu/）功能基因通用数据库，将功能基因数据库提取出来，可根据您的引物序列进行数据库优化，并结合NCBI Taxonomy进行二次检索，得到完备的注释数据库，对您的功能基因序列进行尽可能全面准确的注释。
土壤、水体、肠道内容物、粪便样本等
建议总DNA起始量：>1μg (较纯DNA，无宿主及其他杂质污染)
以下提供可个性化注释的功能基因列表供参考：
Antibiotic resistances
| ACT—Syed Hashsham BEL—Syed Hashsham beta_IS6—Robert Stedtfeld beta_tnpA—Robert Stedtfeld beta_tnpA2—Robert Stedtfeld bet_blaSHV—Robert Stedtfeld bet_tnpA—Robert Stedtfeld CARB—Syed Hashsham cefa_qacEdelta—Robert Stedtfeld chl_cmlA—Robert Stedtfeld CMY—Syed Hashsham cprA—Tamara Tsoi Cole cprB—Tamara Tsoi Cole CTX-M—Syed Hashsham dfra1—Syed Hashsham dfra12—Syed Hashsham FOX—Syed Hashsham gapA—Tim Johnson GES—Syed Hashsham IMI—Syed Hashsham IMP—Syed Hashsham IncW_trwA—Tim Johnson IncW_trwB—Tim Johnson IND—Syed Hashsham intI—Carlos Rodriguez-Minguela intI1_sub1—Tim Johnson intI2—Tim Johnson intI3—Tim Johnson KPC—Syed Hashsham mdh_sub1—Tim Johnson mdh_sub2—Tim Johnson MIR—Syed Hashsham MOX—Syed Hashsham NDM—Syed Hashsham OXA—Syed Hashsham pec_aad2—Robert Stedtfeld PER—Syed Hashsham repA—Tim Johnson SHV—Syed Hashsham SME—Syed Hashsham spec_aad1—Robert Stedtfeld strA—Robert Stedtfeld strB—Robert Stedtfeld strept_aad—Robert Stedtfeld TEM—Syed Hashsham tet1—Robert Stedtfeld tet2—Robert Stedtfeld tet3—Robert Stedtfeld tet31—Syed Hashsham tet4—Robert Stedtfeld tetM—Carlos Rodriguez-Minguela tetQ—Carlos Rodriguez-Minguela tet_sul2—Robert Stedtfeld tetW—Carlos Rodriguez-Minguela vanc_unname—Robert Stedtfeld VEB—Syed Hashsham VIM—Syed Hashsham |
Biodegradation | alkb—Gerben Zylstra/Elyse Rodgers-Vieira benA—Stephan Gantner bph—Gerben Zylstra bphA1—Stephan Gantner bphA2—Stephan Gantner carA—Shoko Iwai dbfA1—Shoko Iwai dxnA—Shoko Iwai dxnA-dbfA1—Tim Johnson npah—Gerben Zylstra p450—Gerben Zylstra/Elyse Rodgers-Vieira ppah—Gerben Zylstra |
Biogeochemical cycles | amoA_AOA—Feifei Liu amoA_AOB—RDP buk—RDP but—RDP cbh1—Cheryl Kuske chb—Fan Yang cooS—Fan Yang cydA—Rachel Morris dsrA—Alexander Loy/Michael Wagner dsrB—Alexander Loy/Michael Wagner exc1—Fan Yang fixN—Rachel Morris glx—Qichao Tu hydA—Fan Yang lcc_ascomycetes—Chris Wright lcc_basidiomycetes—Chris Wright ligE—Ryan Penton lip—Qichao Tu mcrA—Blaz Stres mmoX—Qichao Tu mnp—Qichao Tu nag3—Fan Yang napA—Laurent Philippot narG—Laurent Philippot nifD—RDP nifH—RDP nirA—RDP nirB—RDP nirK—Tracy Teal nirS—Veronica Gruntzig norB—Gesche Braker nosZ—Blaz Stres nosZ_atypical—Robert Sanford nrfA—Joel Klappenbach nrfA_Welsh—Allana Welsh nxrB—RDP phnX—Zarraz Lee pmoA—Tracy K. Teal ppo—Qichao Tu scd2—RDP soxB—Gupta Vadakattu ureA—RDP vp1—Chris Wright xylA—Qichao Tu gene—contributor
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Phylogenetic markers
| EF-Tu—James Kremer fusA—Scott Santos/Howard Ochman gyrB—Zarraz May-Ping Lee ileS—Scott Santos/Howard Ochman lepA—Scott Santos/Howard Ochman leuS—Scott Santos/Howard Ochman pyrG—Scott Santos/Howard Ochman recA—Scott Santos/Howard Ochman recG—Scott Santos/Howard Ochman rplB—Scott Santos/Howard Ochman rpoB—Scott Santos/Howard Ochman |
Metal Cycling
| arsA—PFAM arsB—PFAM arsC—PFAM arsD—PFAM
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Plant Pathogenicity | avrE—James Kremer txtA—RDP txtB—RDP |
Other | baiCD—RDP Staff cagA—Syed Hashsham hcnA—Thierry Janssens phlD—Thierry Janssens phoD—Elizabeth Bent phzA—Thierry Janssens pltA—Thierry Janssens pqsA—Thierry Janssens prnD—Thierry Janssens Spo0A—Jackson Sorenson vacA—Syed Hashsham
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